Growing Fungi and Bacteria of Plants

doorway there argon twain hot and non-virulent bacterium and kingdom Fungi that receive on industrial plants. It is difficult to fill out between the devil without proper review article and diagnosis of the ghoulish plant to shaft whether the bacterium or the fungus in point is the virulent or non-virulent one. Therefore sharp cultures need to be isolated to experience with absolute sure thing which is the causative bacteria or fungus. murphy dextrose nutritious nutrient nutrient nutrient agar-agar-agar is a just nutrient agar for mycelia to thrive on which is present in most fungous moulds. Standard nutrient agar is a general proceeds employ for non-fastidious microorganisms. 2 Aim The post is to isolate fungus kingdom and bacteria colonies from morbid and whole leaves. Materials and Methods Materials used for the experiment was 2 of each measuring stick nutrient agar shell and white potato dextrose agar menage. To remove every epiphytic or saprophyt ic microbes from the plant surface the undulate is superficially sterilise with 100% ethanol. A scalpel to bite the leafSterile body of wet for macerating the leaves. To isolate the fungus kingdom Cut 5 pieces of leaf from the un effectual leaf about the edges of the morbid compass so it contains twain healthy and pathologic parts, beam it on the one potato dextrose agar plate. Then cut 5 pieces from the healthy leaf and place them on the bit dextrose agar plates. To isolate bacteria Macerate both(prenominal)(prenominal) the healthy and pathologic leaves separately in the sterile water and streak it onto the ii separate ideal nutrient agar plate.Incubate all 4 plates at 25? C for up to 7 days. tidings There is emersion on both the healthy and morbid agar plates for both bacteria and kingdom Fungi. For the healthy fungous plate in that location is a single lawsuit of growth which stirs that it is a natural non-pathogenic fungus that grows on plants. On the pathological plants at that place argon 5 diametrical colonies of fungus from the 5 varied pieces of leaves. The one compass has a make it zone where the fungus has hold the growth of the other(a) colonies.On the healthy bacterial plate there is some bacterial growth which move also suggest non-pathogenic bacteria that grows on plants. On the diseased plant bacterial plate there is some bacterial growth merely not a lot and distillery fungal growth. end point The lack of bacterial colonies on the bacterial plate and the fungal growth on the same plate suggests that the disease on the plant is caused by a fungal pathogen and not a bacterial one. There be fungi and bacteria that grow on plants that are not disadvantageous towards the plant. ?